Premium
A simple and reliable method of chromosome banding for prenatal cytogenetics using a bromodeoxyuridine pulse
Author(s) -
Eichenbaum S. Z.,
Krumins E. J.
Publication year - 1983
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.1970030405
Subject(s) - cytogenetics , karyotype , prenatal diagnosis , simple (philosophy) , biology , chromosome , medicine , genetics , pregnancy , fetus , gene , philosophy , epistemology
An extremely simple, fully defined and reliable technique for banding chromosomes in situ is described. The method uses a pulse of bromodeoxyuridine to produce replication pattern banding complete with G‐ and C‐type banding in the same karyotype. This enables immediate resolution of problems otherwise requiring extra subculturing and the application of conventional banding techniques. The value of routine chromosome banding in prenatal cytogenetics using such an economical technique is discussed.