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Preparation of high resolution chromosomes from amniotic fluid cells
Author(s) -
Hoo J. J.,
Jamro Heidi,
Schmutz Sheila,
Lin C. C.
Publication year - 1983
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.1970030315
Subject(s) - ethidium bromide , amniotic fluid , high resolution , resolution (logic) , karyotype , microbiology and biotechnology , biology , chemistry , chromosome , genetics , pregnancy , dna , fetus , geology , computer science , remote sensing , artificial intelligence , gene
A relatively simple method of obtaining high resolution chromosomes from amniotic fluid cells is described. The elongated chromosomes are achieved by adding ethidium bromide (5μg/ml) to the culture 4 1/2 hours before harvesting and the high resolution banding can be produced by usual banding procedures.