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Identification of triploid trophoblast cells in peripheral blood of a woman with a partial hydatidiform molar pregnancy
Author(s) -
van Wijk I. J.,
de Hoon A. C.,
Griffioen S.,
Mulders M. A. M.,
Tjoa M. L.,
van Vugt J. M. G.,
Oudejans C. B. M.
Publication year - 2001
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.190
Subject(s) - trophoblast , peripheral blood , pregnancy , molar pregnancy , partial hydatidiform mole , gynecology , medicine , identification (biology) , obstetrics , molar , andrology , biology , fetus , gestation , placenta , genetics , botany , dentistry
Abstract In a woman with a partial hydatidiform molar pregnancy with 69,XXY karyotype, the presence of male fetal cells of trophoblastic origin was demonstrated in maternal blood by X/Y‐chromosome specific PCR and by immunostaining combined with FISH on two cell populations isolated from maternal blood. Blood was obtained three weeks prior to the detection of fetal demise, at 13 weeks' gestation. Results were confirmed on formalin‐fixed paraffin‐embedded molar tissue, removed at 16 weeks' gestational age for therapeutic reasons. The results indicate that both plasma and cells from maternal peripheral blood might be useful for non‐invasive prenatal diagnosis of fetal aneuploidies, as described in the current case with a partial molar pregnancy. Copyright © 2001 John Wiley & Sons, Ltd.