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Chromosome topology in normal and aneuploid blastomeres from human embryos
Author(s) -
Diblík Jan,
Macek Milan,
Magli M. Cristina,
Krejčí Roman,
Gianaroli Luca
Publication year - 2007
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.1834
Subject(s) - blastomere , aneuploidy , embryo , biology , chromosome , chromosome 21 , genetics , fluorescence in situ hybridization , microbiology and biotechnology , embryogenesis , gene
Objectives To find whether chromosomes 13, 16, 18, 21, 22, X and Y in blastomeres of human embryos are nonrandomly localized, whether their aneuploidy affects their localization and if eventual early inactivation of chromosome X with peripheral localization is present. Methods Relative distances from the nucleus center and edge of 1198 fluorescence in situ hybridization signals in 98 human blastomeres were measured in digital images for comparison with a mathematical model of random distribution in spherical nucleus. Results Comparison with the mathematical model revealed that localization of chromosomes 13, 16, 21, 22, X and Y in normal and aneuploid blastomeres and that of chromosome 18 in normal blastomeres was not significantly different from random distribution. Similarly, chromosome X in blastomeres with more than one X did not appear to have a preferential localization. Only chromosome 18 in aneuploid blastomeres was differently distributed ( p < 0.0001) with a shift to the nuclear periphery ( p = < 0.0001). Conclusions Peripheral localization of chromosome 18 in aneuploid blastomeres is related to embryo aneuploidy. Conversely, a peripheral localization of the inactive X chromosome was not found in blastomeres from 3‐4 day old embryos. These results open the possibility to improve embryo selection after pre‐implantation diagnosis. Copyright © 2007 John Wiley & Sons, Ltd.