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Cell‐free mRNA concentrations of CRH, PLAC1 , and selectin‐P are increased in the plasma of pregnant women with preeclampsia
Author(s) -
Purwosunu Yuditiya,
Sekizawa Akihiko,
Farina Antonio,
Wibowo Noroyono,
Okazaki Shiho,
Nakamura Masamitsu,
Samura Osamu,
Fujito Naoya,
Okai Takashi
Publication year - 2007
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.1780
Subject(s) - preeclampsia , endocrinology , proteinuria , medicine , blood pressure , real time polymerase chain reaction , messenger rna , hormone , eclampsia , placenta , pregnancy , biology , gene , fetus , kidney , biochemistry , genetics
Objective To compare mRNA concentrations of corticotrophin‐releasing hormone (CRH), placenta specific‐1 ( PLAC1 ), and selectin‐P in preeclamptic and normal pregnancies. Methods Peripheral blood samples were obtained from 43 pregnant women with preeclampsia and 41 control subjects. Plasma was harvested from samples and RNA extracted. Plasma RNA was analyzed using reverse transcription polymerase chain reaction (PCR) assay. Median concentrations of CRH, PLAC1 , and selectin‐P mRNA in plasma were compared, to assess possible differences in distribution. Data were also stratified and compared according to clinical severity of preeclampsia. Finally, CRH, PLAC1 , and selectin‐P were plotted against quantitative distributions of blood pressure and proteinuria. Results All markers were differently distributed between cases and controls. Median values in subgroups correlated with severity of preeclampsia. All markers correlated with both. Selectin‐P was identified as the marker with the highest degree of correlation. No correlation was found between any markers in the control group and proteinuria or blood pressure. Conclusion CRH, PLAC1 , and selectin‐P are distributed differently in preeclampsia cases compared to controls and correlate with signs of preeclampsia. Copyright © 2007 John Wiley & Sons, Ltd.