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Evidence of genetic underexpression in chorionic villi samples of euploid fetuses with increased nuchal translucency at 10–11 weeks' gestation
Author(s) -
Farina Antonio,
Volinia Stefano,
Arcelli Diego,
Francioso Francesca,
DeSanctis Paola,
Zucchini Cinzia,
Pilu Gianluigi,
Carinci Paolo,
Morano Danila,
Pittalis M. Carla,
Calderoni Patrizio,
Vagi Sonia,
Rizzo Nicola
Publication year - 2006
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.1373
Subject(s) - biology , chorionic villus sampling , chorionic villi , endocrinology , yolk sac , extracellular matrix , fetus , medicine , andrology , placenta , embryo , microbiology and biotechnology , genetics , prenatal diagnosis , pregnancy
Abstract Objective To retrospectively investigate whether the genetic profile from chorionic villous sampling (CVS) found in euploid fetuses with increased NT differs from matched controls. Study Design We employed cDNA microarray technology to characterize and compare the gene expression profile of chorionic villous tissues (which encompass the trophoblast and inner mesenchymal core) belonging to four singleton male fetuses with increased NT at 10–11 weeks' gestation. A pool of four normal chorionic villous tissues belonging to four respective fetuses, matched for gestational age and gender, was used as controls. Results In euploid fetuses, we found several underexpressed genes, possibly involved in mechanisms associated with the abnormal NT thickness. All these genes are likely to belong to the mesenchymal core of the villus that originates from the extraembryonic mesoderm, and thus might be closely representative of the embryonic genetic profile. They include: (1) genes of embryonic development and differentiation such as Endothelin 3 (EDN3) and secreted frizzled‐related protein 4 (SFRP4); (2) genes of the extracellular matrix (ECM) metabolism such as tissue inhibitor of metalloproteinase1 (TIMP1), and disintegrin‐like and matrix metalloproteinase (MMP) (reprolysin type) with thrombospondin type 1 Motif or ADAMTS2, exostoses (multiple)‐like 1 (EXTL1), heparan sulfate (HS) 6‐O‐sulfotransferase 1 or HS6ST1, fibronectin 1 (FN1) and Integrin Alpha 10 (ITGA10) involved in HS and proteoglycan bio‐synthesis, ECM synthesis and cell‐matrix adhesion; (3) genes involved in vessel formation and differentiation such as angiogenic factor (VG5Q), and in blood pressure control and muscle contraction, like Endothelin 3 or EDN3 and sarcolemma associated protein (SLMAP). Such lower expressions of the villous tissues might be related to an immature genetic profile of the embryo development as well as abnormal regulation of ECM bio‐synthesis and/or improper vessel growth and blood pressure control. Also, the results partially support the theories proposed for NT enlargement such as altered composition of ECM and abnormal/delayed development of the circulatory system. Conclusions Abnormal extraembryonic genetic expression is found at 10–11 weeks' gestation in euploid fetuses with increased NT. If both extra‐ and intraembryonic mesoderms express the same genetic alterations, then microarray analyses on CVS could be used to screen several mesoderm‐derivate anomalies. Copyright © 2006 John Wiley & Sons, Ltd.