Premium
Fluorescent in situ hybridization (FISH) on paraffin‐embedded placental tissues as an adjunct for understanding the etiology of early spontaneous abortion
Author(s) -
Lescoat D.,
Jouan H.,
LoeuilletOlivo L.,
Le Calvé M.
Publication year - 2005
Publication title -
prenatal diagnosis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.956
H-Index - 97
eISSN - 1097-0223
pISSN - 0197-3851
DOI - 10.1002/pd.1132
Subject(s) - etiology , fluorescence in situ hybridization , pathological , products of conception , karyotype , fish <actinopterygii> , pathology , abortion , in situ hybridization , biology , medicine , pregnancy , chromosome , genetics , gene expression , fishery , gene
Objectives An investigation of first‐trimester spontaneous abortions (SAs) for those cases in which karyotype is not available was designed to test the efficiency of fluorescence in situ hybridization (FISH) on paraffin‐embedded tissues combined with pathological examination for understanding the etiology of SAs. Methods Pathological examination of 202 placental tissues from SAs was performed. FISH analysis was then carried out on paraffin‐embedded tissue sections from the same abortion products with probes specific for chromosomes 13, 16, 18, 21, X, Y. Results FISH could be achieved in 196 cases (97%). After pathological analysis alone, the etiology of SAs was evoked in 40 cases. The suspected diagnosis was confirmed by FISH in 26 cases (13.2%). After combined pathological and FISH analysis, the etiology of SAs was identified in 83 from the 196 cases (42.3%) with the probe set used. Conclusion The present study demonstrates the value of FISH on paraffin‐embedded tissues as an adjunct for understanding the etiology of SAs for those cases in which karyotype is not available. Combination of pathological and FISH analysis increases the yield of diagnosis by a factor of 3.2. The results also demonstrate that predictions of the karyotype from pathological examination should be avoided. Copyright © 2005 John Wiley & Sons, Ltd.