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Identification and quantification of C 21 steroidal saponins from radix cynanchi atrati by high‐performance liquid chromatography with evaporative light scattering detection and electrospray mass spectrometric detection
Author(s) -
Liang Mingjin,
Zheng Zhaoguang,
Yuan Ying,
Kong Lingyi,
Shen Yunheng,
Liu Runhui,
Zhang Chuan,
Zhang Weidong
Publication year - 2007
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.998
Subject(s) - chemistry , chromatography , high performance liquid chromatography , radix (gastropod) , analyte , chromatography detector , electrospray , extraction (chemistry) , mass spectrometry , detection limit , tandem mass spectrometry , elution , botany , biology
High‐performance liquid chromatography coupled with electrospray tandem mass spectrometry (HPLC‐ESI/MS) and evaporative light scattering detection (HPLC‐ELSD), respectively, has been performed for the simultaneous identification and quantification of six C 21 steroid saponins, including cynanversicoside A, B, D, G, glaucoside C and glaucogenin C‐3‐ O ‐ β ‐ d ‐thevetopyranoside in Radix Cynanchi Atrati. The extraction of the C 21 steroidal saponins was performed using a B‐811 Buchi Universal Extraction System in Warm Solvent Mode, and the analyte was concentrated by column chromatography before HPLC analysis. The chromatographic separation was performed on an Agilent Zorbax Extend C 18 analytical column efficiently using gradient elution with acetonitrile and water. The method was validated with acceptable linearities ( r > 0.9991) and recoveries (98.2–101.3%). The limits of detection of the C 21 steroid saponins were from 0.2 µg for glaucogenin C‐3‐ O ‐ β ‐ d ‐thevetopyranoside to 0.5 µg for cynanversicoside B. The intra‐ and inter‐day precisions of the method were evaluated and were less than 5.0%. The method was successfully used to analyse 20 batches of Radix Cynanchi Atrati. The content of C 21 steroid saponins in the plant material varied significantly from habitat to habitat, confirming the necessity to control the quality of Radix Cynanchi Atrati during its preparation and application in the clinic. Copyright © 2007 John Wiley & Sons, Ltd.