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Determination of pseudojujubogenin glycosides from Brahmi based on immunoassay using a monoclonal antibody against bacopaside I
Author(s) -
Phrompittayarat Watoo,
Putalun Waraporn,
Tanaka Hiroyuki,
Jetiya Kanchalee,
WittayaAreekul Sakchai,
Ingkaninan Kornkanok
Publication year - 2007
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.996
Subject(s) - chemistry , glycoside , immunogen , monoclonal antibody , saponin , immunoassay , chromatography , splenocyte , antibody , traditional medicine , biochemistry , in vitro , stereochemistry , immunology , biology , pathology , medicine , alternative medicine
Bacopa monnieri (L.) Wettst. (Brahmi) is an Ayurvedic medicinal plant used for centuries as a memory enhancer. Dammarane‐type triterpenoid saponins classified as pseudojujubogenin and jujubogenin glycosides are reported as the active components in this plant. In this study, the monoclonal antibody (MAb) against bacopaside I, a major pseudojujubogenin glycoside found in Brahmi, was produced and characterised. Bacopaside I was conjugated with bovine albumin serum to prepare an immunogen. Hybridomas secreting a monoclonal antibody against bacopaside I was produced by fusing splenocytes from an immunised mouse and SP2/0‐Ag14 myeloma cells. The results showed that the antibodies were raised specifically against pseudojujubonin glycosides. An enzyme‐linked immunosorbant assay (ELISA) using anti‐bacopaside I MAb was performed in the range of 1.95–62.5 ng/mL of bacopaside I, with a limit of detection of 0.5 ng/mL. The method was validated and the practical use of the ELISA for analysing saponin glycosides from Brahmi was demonstrated. Copyright © 2007 John Wiley & Sons, Ltd.