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Analysis of Rhizoma Polygoni Cuspidati by HPLC and HPLC‐ESI/MS
Author(s) -
Yi Tao,
Zhang Hao,
Cai Zongwei
Publication year - 2007
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.993
Subject(s) - chemistry , high performance liquid chromatography , chromatography
An HPLC method with photodiode array detection (PAD) and ESI/MS detection was developed for the qualitative and quantitative analysis of the major chemical constituents of the dried rhizome of Polygonum cuspidatum Sieb. et Zucc. (Rhizoma Polygoni Cuspidati; Chinese name Hu‐Zhang ). Based on the chromatographic separation on an Altima C 18 column using 0.5% aqueous acetic acid and acetonitrile as the mobile phase, nine compounds, including stilbenes, stilbene glucosides, anthraquinones and anthraquinone glucosides, were identified by online ESI/MS analysis and seven were quantified by HPLC‐PAD. A full validation of the method including sensitivity, linearity, repeatability and recovery was conducted. Linear calibration was achieved over the concentration range 1–200 mg/L with R 2 > 0.999, whilst the limits of detection ranged from 0.51 to 1.57 ng. Repeatability was evaluated by intra‐ and inter‐day assays and the RSD value was within 1.79%. Recoveries of the quantified compounds were within the range 96.0–100.1% with RSD values of less than 2.2%. Five samples of Rhizoma Polygoni Cuspidati from different regions were analysed using the developed method. The major constituents piceid, resveratrol, emodin‐8‐ O ‐ β ‐ d ‐glucoside and emodin were selected to provide an index for the quality assessment of the herbal drug. Copyright © 2007 John Wiley & Sons, Ltd.