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Determination of saponins in the kernel cake of Balanites aegyptiaca by HPLC‐ESI/MS
Author(s) -
Chapagain Bishnu P.,
Wiesman Zeev
Publication year - 2007
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.990
Subject(s) - balanites aegyptiaca , chemistry , saponin , chromatography , high performance liquid chromatography , aglycone , electrospray ionization , diosgenin , mass spectrometry , electrospray , methanol , glycoside , stereochemistry , organic chemistry , medicine , alternative medicine , pathology
The kernel cake produced from Balanites aegyptiaca fruit of Israeli origin was analysed for its saponin constituents using high‐performance liquid chromatography–mass spectrometry (HPLC‐MS). The HPLC was equipped with a reversed‐phase C 18 column and a refractive index detector (RID), and elution was isocratic with methanol and water (70:30). The MS system was equipped with electrospray ionisation (ESI). Nine compounds were chromatographically separated, their masses were determined in the negative ion mode and subsequent fragmentation of each component was carried out. From the nine components, six saponins with molecular masses of 1196, 1064, 1210, 1224, 1078 and 1046 Da were identified, with the compound of mass 1210 Da being the main saponin (ca. 36%). Saponins with masses of 1224 and 1046 Da have not been previously reported in B. aegyptiaca. In all saponins, diosgenin was found to be the sole aglycone. This study shows that HPLC‐ESI/MS is a quick and reliable technique for characterizing the saponins from kernel cake of B. aegyptiaca. Copyright © 2007 John Wiley & Sons, Ltd.