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In vivo measurements of changes in pH triggered by oxalic acid in leaf tissue of transgenic oilseed rape
Author(s) -
Zou QiuJu,
Liu ShengYi,
Dong XuYan,
Bi YanHua,
Cao YuanCheng,
Xu Qiao,
Zhao YuanDi,
Chen Hong
Publication year - 2007
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.988
Subject(s) - sclerotinia sclerotiorum , chemistry , brassica , oxalic acid , in vivo , mycelium , transgene , sclerotinia , biochemistry , genetically modified crops , botany , gene , biology , microbiology and biotechnology
Oxalic acid (OA), a non‐host‐specific toxin secreted by Sclerotinia sclerotiorum during pathogenesis, has been demonstrated to be a major phytotoxic and pathogenic factor. Oxalate oxidase (OXO) is an enzyme associated with the detoxification of OA, and hence the introduction of an OXO gene into oilseed rape ( Brassica napus L.) to break down OA may be an alternative way of increasing the resistance of the plant to Sclerotinia sclerotiorum . In order to investigate the activation of OXO in transgenic oilseed rape, a convenient and accessible method was used to monitor changes in pH in response to stress induced by OA. The pH sensor, a platinum microcylinder electrode modified using polyaniline film, exhibited a linear response within the pH range from 3 to 7, with a Nernst response slope of 70 mV/pH at room temperature. The linear correlation coefficient was 0.9979. Changes induced by OA in the pH values of leaf tissue of different oilseed rape species from Brassica napu s L. were monitored in real time in vivo using this electrode. The results clearly showed that the transgenic oilseed rape was more resistant to OA than non‐transgenic oilseed rape. Copyright © 2007 John Wiley & Sons, Ltd.