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Seasonal variations in the isoflavonoids of Radix Puerariae
Author(s) -
Sibao Chen,
Dajian Yang,
Shilin Chen,
Hongxi Xu,
Chan Albert S. C.
Publication year - 2007
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.978
Subject(s) - isoflavonoid , daidzin , chemistry , genistin , pueraria , formononetin , daidzein , radix (gastropod) , puerarin , phytochemical , isoflavones , lobata , chromatography , traditional medicine , flavonoid , botany , genistein , biochemistry , medicine , alternative medicine , pathology , biology , antioxidant
Abstract The variations in isoflavonoid content in Radix Puerariae, the root of Pueraria lobata (Wild) Ohwi, have been examined in order to determine the optimum time to harvest the herb. Total isoflavonoid contents have been analysed by a UV spectrophotometric method whilst HPLC with photodiode array detection has been used to monitor the contents of seven major individual isoflavonoids, namely, 3′‐hydroxypuerarin, puerarin, 3′‐methoxypuerarin, daidzin, genistin, formononetin‐7‐glucoside and daidzein. Ninety‐six samples of Radix Puerariae of different ages and harvested in different months were collected and analysed. The results clearly showed that 3‐year‐old roots harvested in January have the highest yields of isoflavonoid compounds. Moreover, the results obtained by both methods have a strong correlation. This suggested that the simple and fast UV spectrophotometric method could be used for monitoring the phytochemical quality of Radix Puerariae. Copyright © 2007 John Wiley & Sons, Ltd.