Isolation of chlorogenic acids and their derivatives from  Stemona japonica  by preparative HPLC and evaluation of their anti‐AIV (H5N1) activity  in vitro | Zendy

Ge Fan | Zendy; Ke Changqiang | Zendy; Tang Wei | Zendy; Yang Xinzhou | Zendy; Tang Chunping | Zendy; Qin Guowei | Zendy; Xu Rensheng | Zendy; Li Tianxian | Zendy; Chen Xinwen | Zendy; Zuo Jianping | Zendy; Ye Yang | Zendy

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Isolation of chlorogenic acids and their derivatives from Stemona japonica by preparative HPLC and evaluation of their anti‐AIV (H5N1) activity in vitro

Author(s) -

Ge Fan,

Ke Changqiang,

Tang Wei,

Yang Xinzhou,

Tang Chunping,

Qin Guowei,

Xu Rensheng,

Li Tianxian,

Chen Xinwen,

Zuo Jianping,

Ye Yang

Publication year - 2007

Publication title -

phytochemical analysis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.574

H-Index - 72

eISSN - 1099-1565

pISSN - 0958-0344

DOI - 10.1002/pca.974

Subject(s) - chemistry , chlorogenic acid , high performance liquid chromatography , japonica , chromatography , ethyl acetate , in vitro , extraction (chemistry) , ethanol , stereochemistry , organic chemistry , biochemistry , botany , biology

Two chlorogenic acids and five chlorogenic acid derivatives were simultaneously separated and purified from Stemona japonica by preparative high‐performance liquid chromatography. Five of the collected compounds were over 95% pure while the other two compounds were over 90% pure. Their structures were elucidated as 3‐ O ‐feruloylquinic acid ( 1 ), 4‐ O ‐feruloylquinic acid ( 2 ), methyl 3‐ O ‐feruloylquinate ( 3 ), methyl 5‐ O ‐caffeyolquinate ( 4 ), methyl 4‐ O ‐feruloylquinate ( 5 ), ethyl 3‐ O ‐feruloylquinate ( 6 ) and the new compound ethyl 4‐ O ‐feruloylquinate ( 7 ) by UV, NMR and ESI‐MS. All compounds were obtained from Stemona species for the first time, however compounds 6 and 7 are believed to be artefacts from the ethanol extraction. The anti‐AIV (H5N1) activities were evaluated by Neutral Red uptake assay. Compounds 3 and 4 exerted moderate inhibitory effect against AIV (H5N1) in vitro. Copyright © 2007 John Wiley & Sons, Ltd.

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