Premium
Isolation of chlorogenic acids and their derivatives from Stemona japonica by preparative HPLC and evaluation of their anti‐AIV (H5N1) activity in vitro
Author(s) -
Ge Fan,
Ke Changqiang,
Tang Wei,
Yang Xinzhou,
Tang Chunping,
Qin Guowei,
Xu Rensheng,
Li Tianxian,
Chen Xinwen,
Zuo Jianping,
Ye Yang
Publication year - 2007
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.974
Subject(s) - chemistry , chlorogenic acid , high performance liquid chromatography , japonica , chromatography , ethyl acetate , in vitro , extraction (chemistry) , ethanol , stereochemistry , organic chemistry , biochemistry , botany , biology
Two chlorogenic acids and five chlorogenic acid derivatives were simultaneously separated and purified from Stemona japonica by preparative high‐performance liquid chromatography. Five of the collected compounds were over 95% pure while the other two compounds were over 90% pure. Their structures were elucidated as 3‐ O ‐feruloylquinic acid ( 1 ), 4‐ O ‐feruloylquinic acid ( 2 ), methyl 3‐ O ‐feruloylquinate ( 3 ), methyl 5‐ O ‐caffeyolquinate ( 4 ), methyl 4‐ O ‐feruloylquinate ( 5 ), ethyl 3‐ O ‐feruloylquinate ( 6 ) and the new compound ethyl 4‐ O ‐feruloylquinate ( 7 ) by UV, NMR and ESI‐MS. All compounds were obtained from Stemona species for the first time, however compounds 6 and 7 are believed to be artefacts from the ethanol extraction. The anti‐AIV (H5N1) activities were evaluated by Neutral Red uptake assay. Compounds 3 and 4 exerted moderate inhibitory effect against AIV (H5N1) in vitro. Copyright © 2007 John Wiley & Sons, Ltd.