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A rapid TLC autographic method for the detection of glucosidase inhibitors
Author(s) -
Salazar Mario O.,
Furlan Ricardo L. E.
Publication year - 2007
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.971
Subject(s) - chemistry , ferric ion , chromatography , substrate (aquarium) , complex matrix , enzyme , ferric , biochemistry , organic chemistry , oceanography , geology
A new bioautographic assay suitable for the localisation of β ‐glucosidase inhibitors present in a complex matrix is described. Enzyme activity was detected using esculin as the substrate to produce esculetin, which reacts with ferric ion to form a brown complex. Copyright © 2007 John Wiley & Sons, Ltd.
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