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Preparative isolation and purification of geniposide from gardenia fruits by centrifugal partition chromatography
Author(s) -
Kim Chul Young,
Kim Jinwoong
Publication year - 2007
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.958
Subject(s) - chemistry , gardenia jasminoides , chromatography , gardenia , iridoid , ethyl acetate , high performance liquid chromatography , glycoside , partition coefficient , countercurrent chromatography , organic chemistry , medicine , alternative medicine , traditional chinese medicine , pathology
The iridoid glycoside, geniposide was purified by centrifugal partition chromatography (CPC) with a two‐phase solvent system composed of ethyl acetate:isopropanol:water (3:2:5, v/v) from an 80% methanolic extract of fruits of Gardenia jasminoides . Preparative CPC yielded 56.2 mg of geniposide in a one‐step separation of 500 mg of extract, with a purity of 95% as determined by HPLC. Isolated geniposide was identified from its 1 H‐NMR, 13 C‐NMR and MS spectra. Copyright © 2006 John Wiley & Sons, Ltd.