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Influence of plant matrix on microwave‐assisted extraction process. The case of diosgenin extracted from fenugreek ( Trigonella foenum‐graecum L.)
Author(s) -
Kaufmann Béatrice,
Rudaz Serge,
Cherkaoui Samir,
Veuthey JeanLuc,
Christen Philippe
Publication year - 2007
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.954
Subject(s) - trigonella , diosgenin , chemistry , extraction (chemistry) , chromatography , mass spectrometry , gas chromatography–mass spectrometry , matrix (chemical analysis) , yield (engineering) , response surface methodology , botany , organic chemistry , materials science , metallurgy , biology
A focused microwave‐assisted extraction method was developed for the extraction of diosgenin from fenugreek ( Trigonella foenum‐graecum ) seeds, air‐dried and fresh leaves and air‐dried roots. Several experimental parameters were studied, including extraction time, microwave power applied and percentage of 2‐propanol in the extraction mixture as well as their interactions, in order to optimize the extraction efficiency. The two latter parameters were found to be the most important. Response surface modelling was used to predict the extraction yield of diosgenin in selected matrices. The analysis of diosgenin in crude extracts was carried out by capillary gas chromatography–mass spectrometry (GC‐MS). Copyright © 2006 John Wiley & Sons, Ltd.