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Simultaneous determination of ginsenosides in Panax ginseng with different growth ages using high‐performance liquid chromatography–mass spectrometry
Author(s) -
Wang Yi,
Pan JianYang,
Xiao XinYue,
Lin RuiChao,
Cheng YiYu
Publication year - 2006
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.944
Subject(s) - ginseng , chemistry , ginsenoside , chromatography , high performance liquid chromatography , mass spectrometry , medicine , alternative medicine , pathology
Abstract The contents of ginsenosides in Panax ginseng not only vary in different parts of the root, but also exhibit yearly variation. In this study, an HPLC‐MS method was established in order to simultaneously analyse ginsenosides Rb 1 , Rb 2 , Rb 3 , Rc, Rd, Re, Rf, Rg 1 and Rg 2 . The concentration of ginsenosides in the tap root and root fibre were compared and the yearly variations of nine ginsenosides elucidated. The results indicate that the total content of ginsenosides in the main root and the root fibre both attain a maximum level in the fourth year of growth, although the amount in the former is much higher than in the latter. The variation in the content of ginsenosides during a 2–6 year period suggests that cultivated P. Ginseng can be harvested after the fourth year. The current results will provide useful information for the quality control and good agricultural practice farming of ginseng. Copyright © 2006 John Wiley & Sons, Ltd.