Preparative isolation and purification of harpagoside from  Scrophularia ningpoensis  hemsley by high‐speed counter‐current chromatography | Zendy

Tong Shengqiang | Zendy; Yan Jizhong | Zendy; Lou Jianzhong | Zendy

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Preparative isolation and purification of harpagoside from Scrophularia ningpoensis hemsley by high‐speed counter‐current chromatography

Author(s) -

Tong Shengqiang,

Yan Jizhong,

Lou Jianzhong

Publication year - 2006

Publication title -

phytochemical analysis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.574

H-Index - 72

eISSN - 1099-1565

pISSN - 0958-0344

DOI - 10.1002/pca.938

Subject(s) - chemistry , countercurrent chromatography , chromatography , high performance liquid chromatography , partition coefficient , glycoside , organic chemistry

The bioactive component harpagoside was successfully separated from the crude extract of Scrophularia ningpoensis Hemsley by one‐step purification using high‐speed counter‐current chromatography (HSCCC). A two‐phase solvent system containing n ‐butanol:ethyl acetate:water (1:9:10) was selected following consideration of the partition coefficient of the target compound. A 276 mg quantity of the crude extract was loaded onto a 250 mL HSCCC column and yielded 11 mg harpagoside at over 97% purity. The chemical structure of harpagoside was determined by HPLC‐ESI/MS and 1H‐NMR. Copyright © 2006 John Wiley & Sons, Ltd.

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