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On‐line identification of further flavone C ‐ and O ‐glycosides from sugarcane ( Saccharum officinarum L., Gramineae) by HPLC‐UV‐MS
Author(s) -
Colombo Renata,
Yariwake Janete H.,
Queiroz Emerson F.,
Ndjoko Karine,
Hostettmann Kurt
Publication year - 2006
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.923
Subject(s) - chemistry , saccharum , poaceae , glycoside , high performance liquid chromatography , saccharum officinarum , botany , chromatography , stereochemistry , biology
HPLC‐UV‐MS (APCI‐MS/MS and CID/MS) was utilised for the identication of eight additional avone glycosides from sugarcane ( Saccharum ofcinarum L., Gramineae) extracts of bagasse, leaves and juice (‘garapa’). Relevant information about substitution patterns was obtained through UV detection using post‐column addition of shift reagents, while tandem MS provided structural information conrming the proposed structures of the C ‐glycosides vitexin, orientin, luteolin‐8‐ C ‐(rhamnosylglucoside), 4′,5′‐dimethyl‐luteolin‐8‐ C ‐glycoside and the isomeric pair schaftoside–isoschaftoside, besides the O ‐glycosides tricin‐7‐ O ‐neohesperidoside and tricin‐7‐ O ‐glycoside. Copyright © 2006 John Wiley & Sons, Ltd.