Premium
A combined HPLC‐UV and HPLC‐MS method for the identification of lignans and its application to the lignans of Linum usitatissimum L. and L. bienne Mill.
Author(s) -
Schmidt Thomas J.,
Hemmati Shiva,
Fuss Elisabeth,
Alfermann A. Wilhelm
Publication year - 2006
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.918
Subject(s) - linum , chemistry , chromatography , high performance liquid chromatography , lignan , botany , stereochemistry , biology
A combined HPLC‐UV/PAD and HPLC‐ESI/MS method allowing the fast detection and identification/structural characterisation of lignans of different structural subclasses is described. Twenty‐four lignans of different skeletal types were analysed and the combined information derived from their UV and ESI/MS spectra led to the identification of group characteristics that can be used to establish the structure of unknown lignans in plant samples. This method was successfully applied to the identification of lignans in crude extracts of Linum usitatissimum L. and L. bienne Mill. Copyright © 2006 John Wiley & Sons, Ltd.