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Development of an HPLC‐PAD‐MS assay for the identification and quantification of major phenolic edelweiss ( Leontopodium alpium Cass.) constituents
Author(s) -
Schwaiger Stefan,
Seger Christoph,
Wiesbauer Bettina,
Schneider Peter,
Ellmerer Ernst P.,
Sturm Sonja,
Stuppner Hermann
Publication year - 2006
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.917
Subject(s) - chemistry , luteolin , apigenin , glucoside , chromatography , high performance liquid chromatography , column chromatography , stereochemistry , quercetin , flavonoid , biochemistry , medicine , alternative medicine , pathology , antioxidant
Abstract The analytical assessment of edelweiss ( Leontopodium alpinum ) herb extracts, used in traditional alpine medicine, has resulted in the development of a HPLC‐PAD‐MS method that allows baseline separation of almost all constituents. Peak assignment of 14 analytes was achieved by comparison of retention times, UV and mass spectra with those of reference compounds either commercially available (luteolin, apigenin and chlorogenic acid) or isolated from edelweiss plants by column chromatography. Ten of the isolated analytes were identified as the known natural products: quercetin‐3‐ O ‐ β ‐ d ‐glucoside, luteolin‐7‐ O ‐ β ‐ d ‐glucoside, luteolin‐3′‐ O ‐ β ‐ d ‐glucoside, luteolin‐4′‐ O ‐ β ‐ d ‐glucoside, apigenin‐7‐ O ‐ β ‐ d ‐glucoside, 6‐hydroxy‐luteolin‐7‐ O ‐ β ‐ d ‐glucoside, luteolin‐7,4′‐di‐ O ‐ β ‐ d ‐glucoside, chrysoeriol‐7‐ O ‐ β ‐ d ‐glucoside, leontopodic acid and 3,5‐dicaffeolyquinic acid. One analyte, 3,4,5‐tri‐( E )‐caffeoly‐d‐glucaric acid proved to be a new natural product and was named leontopodic acid B. Structure elucidation was carried out by means of MS and NMR spectroscopy in all cases. The aerial plant parts of L. alpinum (capitula, inflorescence leaves, stems, stem leaves and leaves of the basal rosette) showed variable amounts of the above‐mentioned constituents, although qualitative differences were not observable. Copyright © 2006 John Wiley & Sons, Ltd.