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Quantitative analysis of polymeric proanthocyanidins in birch leaves with normal‐phase HPLC
Author(s) -
Karonen Maarit,
Ossipov Vladimir,
Sinkkonen Jari,
Loponen Jyrki,
Haukioja Erkki,
Pihlaja Kalevi
Publication year - 2006
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.898
Subject(s) - proanthocyanidin , chemistry , high performance liquid chromatography , chromatography , quantitative analysis (chemistry) , food science , polyphenol , biochemistry , antioxidant
The proanthocyanidin composition and content in the leaves of nine birch species ( Betula albosinensis , B . ermanii , B . maximowicziana , B . nana , B . papyrifera , B . pendula , B . platyphylla , B . pubescens , and B . pubescens ssp. czerepanovii ) were studied with different methods including colorimetric assay, HPLC coupled with PAD or ESI/MS and NMR. Total proanthocyanidin content was determined using the acid butanol assay. A normal phase‐HPLC method was applied for the analysis of polymeric proanthocyanidins. The content of polymeric proanthocyanidins was estimated from a late eluting peak in the chromatogram. With this HPLC method, quantitative analysis of polymeric proanthocyanidins could be performed directly from leaf extracts: no additional purification or preparation steps were required. It was shown that birch leaves contained mainly polymeric proanthocyanidins with a degree of polymerisation greater than 10. Total proanthocyanidin content (expressed as dry weight) was found to vary from 44 mg/g ( B . papyrifera ) to 145 mg/g ( B . nana ), and polymeric proanthocyanidin content from 39 mg/g ( B . pendula ) to 119 mg/g ( B . nana ). Copyright © 2006 John Wiley & Sons, Ltd.