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A novel method to convert triptolide into tripchlorolide in Tripterygium wilfordii
Author(s) -
Li Baozhi,
Bai Jie,
Yang Gengliang,
Li Zhiwei,
Wang Lijuan,
Chen Yi
Publication year - 2006
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.896
Subject(s) - triptolide , tripterygium wilfordii , chemistry , hydrochloric acid , chromatography , acetic acid , high performance liquid chromatography , organic chemistry , biochemistry , medicine , apoptosis , alternative medicine , pathology
A new method has been established to convert triptolide (1) into tripchlorolide (2) directly in the Chinese herbal drug Tripterygium wilfordii Hook F. and the influences of reaction times and pH values have been investigated. It was found that 1 could be most efficiently converted into 2 using a hydrochloric acid–acetic acid system. An HPLC method was devised in order to monitor the conversion and ESI‐MS was used to identify the synthetic product 2. The sensitivity of the assay was sufficient to monitor the conversion of the main active components in T. wilfordii. Copyright © 2006 John Wiley & Sons, Ltd.