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Dereplication of pentacyclic triterpenoids in plants by GC‐EI/MS
Author(s) -
Gu JianQiao,
Wang Yuehong,
Franzblau Scott G.,
Montenegro Gloria,
Timmermann Barbara N.
Publication year - 2006
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.892
Subject(s) - chemistry , ursolic acid , betulinic acid , oleanolic acid , triterpenoid , terpene , gas chromatography–mass spectrometry , triterpene , chromatography , stereochemistry , mass spectrometry , medicine , genetics , biology , alternative medicine , pathology
Three common plant‐derived pentacyclic triterpenoids, oleanolic acid ( 1 ), betulinic acid ( 2 ) and ursolic acid ( 3 ), have been found to exhibit moderate anti‐tubercular activity in a microplate alamar blue assay. In order to facilitate the discovery of novel anti‐tubercular leads with diverse chemical structures, a new and rapid GC‐EI/MS method was developed simultaneously and unambiguously to dereplicate 1–3 as their methyl esters with limits of detection of 25.6, 26.9 and 26.8 ng, respectively. Copyright © 2006 John Wiley & Sons, Ltd.