A rapid TLC autographic method for the detection of xanthine oxidase inhibitors and superoxide scavengers | Zendy

Ramallo I. Ayelen | Zendy; Zacchino Susana A. | Zendy; Furlan Ricardo L. E. | Zendy

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A rapid TLC autographic method for the detection of xanthine oxidase inhibitors and superoxide scavengers

Author(s) -

Ramallo I. Ayelen,

Zacchino Susana A.,

Furlan Ricardo L. E.

Publication year - 2005

Publication title -

phytochemical analysis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.574

H-Index - 72

eISSN - 1099-1565

pISSN - 0958-0344

DOI - 10.1002/pca.874

Subject(s) - chemistry , superoxide , formazan , xanthine oxidase , enzyme , biochemistry , oxidase test , xanthine , chromatography

A new bioautographic assay suitable for the localization of xanthine oxidase inhibitors and superoxide radical scavengers present in a complex matrix is described. Enzyme activity is detected by reaction of superoxide radicals with nitroblue tetrazolium to form a blue formazan salt. Both activities can be differentiated using a non‐enzymatic version of the autographic assay wherein superoxide is chemically generated. Copyright © 2005 John Wiley & Sons, Ltd.

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