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A rapid TLC autographic method for the detection of xanthine oxidase inhibitors and superoxide scavengers
Author(s) -
Ramallo I. Ayelen,
Zacchino Susana A.,
Furlan Ricardo L. E.
Publication year - 2005
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.874
Subject(s) - chemistry , superoxide , formazan , xanthine oxidase , enzyme , biochemistry , oxidase test , xanthine , chromatography
A new bioautographic assay suitable for the localization of xanthine oxidase inhibitors and superoxide radical scavengers present in a complex matrix is described. Enzyme activity is detected by reaction of superoxide radicals with nitroblue tetrazolium to form a blue formazan salt. Both activities can be differentiated using a non‐enzymatic version of the autographic assay wherein superoxide is chemically generated. Copyright © 2005 John Wiley & Sons, Ltd.