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Characterisation of the phenolic profile of Boerhaavia diffusa L. by HPLC‐PAD‐MS/MS as a tool for quality control
Author(s) -
Ferreres Federico,
Sousa Carla,
Justin Mulangu,
Valentão Patrícia,
Andrade Paula B.,
Llorach Rafael,
Rodrigues Alírio,
Seabra Rosa M.,
Leitão Anabela
Publication year - 2005
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.869
Subject(s) - chemistry , quercetin , kaempferol , flavonols , chromatography , high performance liquid chromatography , phenolic acid , biochemistry , antioxidant
Phenolic acids and flavonols of nine leaf and three root samples of Boerhaavia diffusa L., collected at different locations and subjected to several drying procedures, were characterised by reversed‐phase HPLC‐PADESI/MS for the first time. Ten phenolic compounds were identified: 3,4‐dihydroxy‐5‐methoxycinnamoyl‐rhamnoside, quercetin 3‐ O ‐rhamnosyl(1→6)galactoside (quercetin 3‐ O ‐robinobioside), quercetin 3‐ O ‐(2″‐rhamnosyl)‐robinobioside, kaempferol 3‐ O ‐(2″‐rhamnosyl)‐robinobioside, 3,5,4′‐trihydroxy‐6,7‐dimethoxyflavone 3‐ O ‐galactosyl(1→2)glucoside [eupalitin 3‐ O ‐galactosyl(1→2)glucoside], caffeoyltartaric acid, kaempferol 3‐ O ‐robinobioside, eupalitin 3‐ O ‐galactoside, quercetin and kaempferol. Quantification was achieved by HPLC‐PAD and two phenolic patterns were found for the leaves, in which quercetin 3‐ O ‐robinobioside or quercetin 3‐ O ‐(2″‐rhamnosyl)‐robinobioside was the major compound. Caffeoyltartaric acid was only present in the root material where it represented the main phenolic constituent. The results obtained demonstrated that the geographical origin (particularly the nature of the soil), but not the drying process, influences the phenolic composition. Copyright © 2005 John Wiley & Sons, Ltd.