Rapid detection of glycyrrhizin by immunochromatographic assay | Zendy

Putalun Waraporn | Zendy; Tanaka Hiroyuki | Zendy; Shoyama Yukihiro | Zendy

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Rapid detection of glycyrrhizin by immunochromatographic assay

Author(s) -

Putalun Waraporn,

Tanaka Hiroyuki,

Shoyama Yukihiro

Publication year - 2005

Publication title -

phytochemical analysis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.574

H-Index - 72

eISSN - 1099-1565

pISSN - 0958-0344

DOI - 10.1002/pca.862

Subject(s) - chemistry , glycyrrhizin , detection limit , chromatography , immunoassay , monoclonal antibody , colloidal gold , reagent , antibody , nanoparticle , nanotechnology , medicine , materials science , immunology , biology

An immunochromatographic assay was developed for detecting glycyrrhizin (1). The qualitative assay is based on a competitive immunoassay using anti‐1 monoclonal antibody (MAb) and a detector reagent that contains colloidal gold particles coated with anti‐1 MAb. The immunochromatographic strip test, which has a detection limit of 250 ng/mL, is useful as a rapid screening method for detecting glycyrrhizin in plants, biological fluids and food samples. Copyright © 2005 John Wiley & Sons, Ltd.

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