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Rapid detection of glycyrrhizin by immunochromatographic assay
Author(s) -
Putalun Waraporn,
Tanaka Hiroyuki,
Shoyama Yukihiro
Publication year - 2005
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.862
Subject(s) - chemistry , glycyrrhizin , detection limit , chromatography , immunoassay , monoclonal antibody , colloidal gold , reagent , antibody , nanoparticle , nanotechnology , medicine , materials science , immunology , biology
An immunochromatographic assay was developed for detecting glycyrrhizin (1). The qualitative assay is based on a competitive immunoassay using anti‐1 monoclonal antibody (MAb) and a detector reagent that contains colloidal gold particles coated with anti‐1 MAb. The immunochromatographic strip test, which has a detection limit of 250 ng/mL, is useful as a rapid screening method for detecting glycyrrhizin in plants, biological fluids and food samples. Copyright © 2005 John Wiley & Sons, Ltd.