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Rapid identification of vinca alkaloids by direct‐injection electrospray ionisation tandem mass spectrometry and confirmation by high‐performance liquid chromatography–mass spectrometry
Author(s) -
Zhou Hui,
Tai Yuanpo,
Sun Cuirong,
Pan Yuanjiang
Publication year - 2005
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.852
Subject(s) - chemistry , vindoline , catharanthus roseus , vinca , chromatography , mass spectrometry , tandem mass spectrometry , electrospray , electrospray ionization , sanguinarine , apocynaceae , alkaloid , vinblastine , stereochemistry , traditional medicine , biochemistry , medicine , botany , surgery , chemotherapy , biology
A simple and rapid method for the identification of Vinca alkaloids from a crude extract of Catharanthus roseus G. Don (Apocynaceae) by direct‐injection electrospray ionisation (ESI) and tandem mass spectrometry (MS/MS) has been developed. The alkaloids vindoline, vindolidine, vincristine and vinblastine were evaluated in a commercial extract of C. roseus using this method. Catharanthine and its isomers 19 S ‐vindolinine and vindolinine were detected in the commercial product by direct injection ESI/MS/MS and confirmed by preparation and by HPLC‐ESI/MS. For the characterisation of different fragment fingerprints, ESI/MS/MS is a sensitive, rapid and convenient technique by which to identify some constituents in complex and mixed plant extracts. Copyright © 2005 John Wiley & Sons, Ltd.