Rapid identification of vinca alkaloids by direct‐injection electrospray ionisation tandem mass spectrometry and confirmation by high‐performance liquid chromatography–mass spectrometry | Zendy

Zhou Hui | Zendy; Tai Yuanpo | Zendy; Sun Cuirong | Zendy; Pan Yuanjiang | Zendy

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Rapid identification of vinca alkaloids by direct‐injection electrospray ionisation tandem mass spectrometry and confirmation by high‐performance liquid chromatography–mass spectrometry

Author(s) -

Zhou Hui,

Tai Yuanpo,

Sun Cuirong,

Pan Yuanjiang

Publication year - 2005

Publication title -

phytochemical analysis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.574

H-Index - 72

eISSN - 1099-1565

pISSN - 0958-0344

DOI - 10.1002/pca.852

Subject(s) - chemistry , vindoline , catharanthus roseus , vinca , chromatography , mass spectrometry , tandem mass spectrometry , electrospray , electrospray ionization , sanguinarine , apocynaceae , alkaloid , vinblastine , stereochemistry , traditional medicine , biochemistry , medicine , botany , surgery , chemotherapy , biology

A simple and rapid method for the identification of Vinca alkaloids from a crude extract of Catharanthus roseus G. Don (Apocynaceae) by direct‐injection electrospray ionisation (ESI) and tandem mass spectrometry (MS/MS) has been developed. The alkaloids vindoline, vindolidine, vincristine and vinblastine were evaluated in a commercial extract of C. roseus using this method. Catharanthine and its isomers 19 S ‐vindolinine and vindolinine were detected in the commercial product by direct injection ESI/MS/MS and confirmed by preparation and by HPLC‐ESI/MS. For the characterisation of different fragment fingerprints, ESI/MS/MS is a sensitive, rapid and convenient technique by which to identify some constituents in complex and mixed plant extracts. Copyright © 2005 John Wiley & Sons, Ltd.

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