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Analysis of aristolochic acids and analogues in medicinal plants and their commercial products by HPLC‐PAD‐ESI/MS
Author(s) -
Wei Feng,
Cheng XianLong,
Ma LinYun,
Jin WenTao,
Schaneberg Brian T.,
Khan Ikhlas A.,
Lin RuiChao
Publication year - 2005
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.849
Subject(s) - aristolochic acid , chemistry , chromatography , high performance liquid chromatography , electrospray , mass spectrometry , electrospray ionization , genetics , biology
An HPLC‐UV‐MS method for the analysis of aristolochic acids A, B, C and D, 7‐OH‐aristolochic acid A, and aristolic acid in a number of plant materials and their commercial products has been developed. HPLC with photodiode array detection and electrospray ionisation‐MS in the selected ion monitoring mode allowed the identification of the target compounds and increased the selectivity of complex analyses such as those associated with multi‐botanical preparations. The presented method was used to analyse 10 plant samples and six commercial products that possibly contained aristolochic acids. The resulting chromatographic profiles of the samples were significantly different from each other, and the method was directly transferred to HPLC‐MS, which was used to confirm the presence of the six aristolochic acids mentioned above. Copyright © 2005 John Wiley & Sons, Ltd.