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Determination of aflatoxins in medicinal herbs by HPLC. An efficient method for routine analysis
Author(s) -
GómezCatalán Jesús,
Piqué Ester,
Falcó Gemma,
Borrego Natividad,
Rodamilans Miquel,
Llobet Juan M.
Publication year - 2005
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.845
Subject(s) - chemistry , repeatability , chromatography , aflatoxin , reproducibility , extraction (chemistry) , high performance liquid chromatography , detection limit , medicinal herbs , sample preparation , traditional medicine , food science , medicine
A fast and easy to perform method for the routine determination of aflatoxins in medicinal herbs was developed. The described method involves a single‐step extraction with a non‐chlorinated solvent, an immunoaffinity clean‐up and HPLC with fluorescence detection. Whilst assays with naturally contaminated and with spiked samples of several herbs showed that the recoveries were somewhat low and dependent on the kind of sample and the degree of grinding, the intra‐batch reproducibility was good, allowing a reliable quantitation by the standard‐addition method. Good linearity, repeatability and accuracy were demonstrated in assays involving several medicinal herbs. The limit of quantivation was of the order of 0.05–0.1 ng/g, being dependent of the species analysed, and the method required no tedious concentration or back‐extraction steps. Copyright © 2005 John Wiley & Sons, Ltd.