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Isolation of secondary metabolites from roots of Plumbago auriculata Lam. by countercurrent chromatography
Author(s) -
de Paiva Selma Ribeiro,
Figueiredo Maria Raquel,
Kaplan Maria Auxiliadora Coelho
Publication year - 2005
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.841
Subject(s) - chemistry , chromatography , isolation (microbiology) , countercurrent chromatography , countercurrent distribution , column chromatography , microbiology and biotechnology , biology
Centrifugal liquid–liquid partition chromatography presents significant advantages for the separation and purification of plant metabolites owing to the short operational time of the process and the elimination of possible irreversible adsorption of compounds. The crude chloroform extract from roots of Plumbago auriculata was analysed by countercurrent chromatography using hexane:ethyl acetate:methanol:water (40:10:10:2, v/v) as solvent system. The isolation of the naphthoquinones plumbagin and epi ‐isoshinanolone, the steroids sitosterol and 3‐ O ‐glucosylsitosterol, plumbagic and palmitic acids was easily achieved. Naphthoquinones are typical components of Plumbago species and they show interesting biological activities. Copyright © 2005 John Wiley & Sons, Ltd.