Premium
Development and validation of a method for the quantitative determination of aflatoxin contaminants in Maytenus ilicifolia by HPLC with fluorescence detection
Author(s) -
Braga Sayonara Ma Lia Fook Meira,
de Medeiros Francinalva Dantas,
de Jesus Oliveira Eduardo,
Macedo Rui Oliveira
Publication year - 2005
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.837
Subject(s) - chemistry , aflatoxin , chromatography , high performance liquid chromatography , contamination , fluorescence , mycotoxin , food science , physics , quantum mechanics , ecology , biology
A method for the quantification of aflatoxins B 1 , G 1 , B 2 and G 2 in the medicinal herb Maytenus ilicifolia was developed and validated. The method used immunoaffinity columns for sample clean‐up and HPLC with fluorescence detection without any derivatisation step. The method showed good inter‐day accuracy (bias values in the range 4.5–10.7%) and precision (5–16% RSD) when applied to the determination of levels of aflatoxins ranging from 7 to 20 ppb in the plant material. The detection limits for samples of the plant material spiked with aflatoxins were 3.5 ng/g for B 1 and G 1 and 0.1 ng/g for B 2 and G 2 . The method was successfully applied to commercial samples of Maytenus ilicifolia for the screening of aflatoxin contaminants. Copyright © 2005 John Wiley & Sons, Ltd.