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Isolation and purification of closely related Citrus limonoid glucosides by flash chromatography
Author(s) -
Raman Girija,
Cho Minhee,
Brodbelt Jennifer S.,
Patil Bhimanagouda S.
Publication year - 2005
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.835
Subject(s) - chemistry , limonoid , chromatography , flash (photography) , column chromatography , isolation (microbiology) , organic chemistry , art , microbiology and biotechnology , visual arts , biology
Abstract Several citrus limonoid glycosides have proved to be particularly difficult to purify using conventional techniques. A reversed‐phase flash chromatographic technique has been developed for the separation and isolation of the closely related limonoid glucosides, nomilin 17‐ β ‐ d ‐glucopyranoside and nomilinic acid 17‐ β ‐ d ‐glucopyranoside, with confirmation of their identities by electrospray ionisation mass spectrometry. Furthermore, the semipurification of the mixture of glucosides enriched with flavanone glucosides such as naringin, narirutin and other limonoid glucosides was obtained. The closely eluting glucosides were successfully separated to achieve a good yield and purity of 93%. Copyright © 2005 John Wiley & Sons, Ltd.