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Secondary metabolites in in vitro cultured plants of the genus Drosera
Author(s) -
Marczak Ł.,
Kawiak A.,
Łojkowska E.,
Stobiecki M.
Publication year - 2005
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.833
Subject(s) - chemistry , plumbagin , high performance liquid chromatography , flavonols , secondary metabolite , in vitro , chromatography , extraction (chemistry) , botany , flavonoid , biochemistry , antioxidant , biology , gene
Extracts from plantlets of different species of the genus Drosera , grown as in vitro cultures, were evaluated for the level of phenolic secondary metabolites from the group of naphthoquinones and flavonols. The profiles of natural products in the extracts obtained from different species were monitored by HPLC with UV detection at 260 and 330 nm. On the basis of the data obtained, Drosera binata , the species with the highest amount of plumbagin, was selected for further studies. The most effective method of extraction of quinones was established and the composition of phenolic secondary metabolites in the tissues was determined. For the identification of phenolic compounds, HPLC‐UV and HPLC‐ESI/MS were applied. Copyright © 2005 John Wiley & Sons, Ltd.