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Determination of diarylheptanoids from Alpinia officinarum (lesser galangal) by HPLC with photodiode array and electrochemical detection
Author(s) -
Liu Zhihua,
Sang Shengmin,
Hartman Thomas G.,
Ho ChiTang,
Rosen Robert T.
Publication year - 2005
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.827
Subject(s) - diarylheptanoids , chemistry , chromatography , high performance liquid chromatography , rhizome , detection limit , stereochemistry , traditional medicine , medicine
Normal‐phase column chromatography followed by semi‐preparative reversed‐phase HPLC has been used to isolate, from the rhizomes of Alpinia officinarum , five diarylheptanoids identified as 5‐hydroxy‐7‐(4″‐hydroxy‐3″‐methoxyphenyl)‐1‐phenyl‐3‐heptanone, 5‐methoxy‐7‐(4″‐hydroxy‐3″‐methoxyphenyl)‐1‐phenyl‐3‐heptanone, 7‐(4″‐hydroxyphenyl)‐1‐phenylhept‐4‐en‐3‐one, 7‐(4″‐hydroxy‐3″‐methoxyphenyl)‐1‐phenyl‐hept‐4‐en‐3‐one, 1,7‐diphenylhept‐4‐en‐3‐one. The levels of these five diarylheptanoids in root material were determined quantitatively by HPLC with UV detection and the assay methods so developed were simple, rapid and accurate. Four of the diarylheptanoids could also be detected by HPLC with electrochemical detection (ECD) in the oxidative mode, and ECD was found to have a higher sensitivity than photodiode array detection. Copyright © 2005 John Wiley & Sons, Ltd.