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An HPLC‐PAD method to analyse flavonoid glycosides and styrylpyrones from Cryptocarya species (lauraceae)
Author(s) -
Nehme Cláudia J.,
Bastos Wagner L.,
de Araújo Adelson J.,
Cavalheiro Alberto J.
Publication year - 2005
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.818
Subject(s) - chemistry , chromatography , high performance liquid chromatography , lauraceae , flavonoid , glycoside , extraction (chemistry) , sample preparation , reproducibility , botany , organic chemistry , biology , antioxidant
Abstract An HPLC‐PAD method has been developed in order to evaluate simultaneously the main secondary metabolites, flavonoid glycosides and styrylpyrones, of leaves of Cryptocarya moschata . The sample preparation, consisting of extraction, liquid–liquid extraction and centrifugation, requires minimum sample manipulation but produces high yields with reproducibility, selectivity and simplicity. HPLC on a C 18 column presents each class of metabolites grouped and with good resolution of the main compounds. The experimental conditions can be used to study inter‐ and intra‐specific variability of secondary metabolites in Cryptocarya spp. Copyright © 2005 John Wiley & Sons, Ltd.