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Isolation and identification of arctiin and arctigenin in leaves of burdock ( Arctium lappa L.) by polyamide column chromatography in combination with HPLC‐ESI[sol ]MS
Author(s) -
Liu Shiming,
Chen Kaoshan,
Schliemann Willibald,
Strack Dieter
Publication year - 2005
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.816
Subject(s) - arctium lappa , chemistry , chromatography , high performance liquid chromatography , polyamide , column chromatography , traditional medicine , organic chemistry , medicine
A simple method involving polyamide column chromatography in combination with HPLC‐PAD and HPLC‐ESI[sol ]MS for isolating and identifying two kinds of lignans, arctiin and arctigenin, in the leaves of burdock ( Arctium lappa L.) has been established. After extraction of burdock leaves with 80% methanol, the aqueous phase of crude extracts was partitioned between water and chloroform and the aqueous phase was fractionated on a polyamide glass column. The fraction, eluting with 100% methanol, was concentrated and gave a white precipitate at 4°C from which two main compounds were purified by semi‐preparative HPLC. In comparison with the UV and ESI‐MS spectra and the HPLC retention time of authentic standards, the compounds were determined to be arctiin and arctigenin. The extraction[sol ]separation technique was validated using an internal standard method. Copyright © 2005 John Wiley & Sons, Ltd.