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Quality control of roots of Eleutherococcus senticosus by HPLC
Author(s) -
Apers Sandra,
Naessens Tania,
Van Miert Sabine,
Pieters Luc,
Vlietinck Arnold
Publication year - 2005
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.811
Subject(s) - chemistry , chromatography , phosphoric acid , ferulic acid , repeatability , high performance liquid chromatography , extraction (chemistry) , gradient elution , organic chemistry
An HPLC method based on several known methods for the determination of eleutherosides B and E was developed, optimised and validated in terms of linearity, precision (repeatability and intermediate precision on different days and at different concentration levels) and accuracy (recovery). The extraction procedure, the extraction solvent and the extraction yield were evaluated and optimised. A reversed‐phase RP‐18 column gradient eluted with a two‐phase system consisting of phosphoric acid:water (0.5:99.5) and acetonitrile was used to evaluate the samples; detection was at 220 nm. Although eleutherosides B and E are commercially available, they are very costly, and therefore ferulic acid was chosen as external standard. The correction factors for the response of ferulic acid against both eleutherosides were determined and validated. This method, accepted by the European Pharmacopoeia Commission, will be included in the monograph on Eleutherococcus senticosus roots to assay the content of eleutherosides B and E. Copyright © 2005 John Wiley & Sons, Ltd.