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Discriminating the xanthones in an extract of Swertia franchetiana by retention parameters
Author(s) -
Longxing Wang,
LeFeng Zhang,
Shisheng Wang,
Hongbin Xiao,
Xinmiao Liang
Publication year - 2005
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.807
Subject(s) - xanthone , chemistry , chromatography , retention time , high performance liquid chromatography , methanol , stereochemistry , organic chemistry
The quantitative structure—retention relationship is one of the most actively studied topics in the eld of chromatography. In this paper, retention parameters of components were used to discriminate the xanthones in a methanol extract of Swertia franchetiana . The extract was analysed by HPLC under two different multistage linear gradient conditions and the retention parameters calculated from these retention data. It was found that the retention parameters of xanthones are in a specic region in the plot of log k w vs. S and the xanthones in the extract could be distinguished from other components by this feature. Furthermore, xanthone aglycones and xanthone glucosides could also be discriminated by retention parameters. Copyright © 2005 John Wiley & Sons, Ltd.