Premium
HPLC quantification of uncarine D and the anti‐plasmodial activity of alkaloids from leaves of Mitragyna inermis (Willd.) O. Kuntze
Author(s) -
Fiot Julien,
Baghdikian Béatrice,
Boyer Laurent,
Mahiou Valérie,
Azas Nadine,
Gasquet Monique,
TimonDavid Pierre,
Balansard Guy,
Ollivier Evelyne
Publication year - 2005
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.806
Subject(s) - chemistry , oxindole , high performance liquid chromatography , indole test , chromatography , traditional medicine , rubiaceae , alkaloid , stereochemistry , botany , organic chemistry , medicine , catalysis , biology
An efcient system for the analysis of the total alkaloids extracted from leaves of Mitragyna inermis (Willd.) O. Kuntze (Rubiaceae) by HPLC using a reversed‐phase column is described. The chromatographic conditions allowed the separation of indole and oxindole alkaloids in leaf extracts, and the quantication of uncarine D in samples collected in Burkina Faso and Mali. The HPLC method described was validated for its specicity, linearity and precision using an internal standard (naphthalene). The concentrations of uncarine D in various extracts were compared with their in vitro anti‐plasmodial activity. The anti‐proliferative activity on chloroquine‐resistant strain (W 2 ) of Plasmodium falciparum was not correlated with the concentration of uncarine D in leaves. Copyright © 2005 John Wiley & Sons, Ltd.