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Quantitative determination of the major saponin mixture bacoside A in Bacopa monnieri by HPLC
Author(s) -
Deepak M.,
Sangli G. K.,
Arun P. C.,
Amit A.
Publication year - 2005
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.805
Subject(s) - bacopa monnieri , chemistry , chromatography , saponin , high performance liquid chromatography , traditional medicine , medicine , alternative medicine , pathology
Bacoside A, the putative bioactive component of the Indian medicinal plant Bacopa monnieri , was found to be a mixture of saponins with bacoside A 3 (1), bacopaside II (2), jujubogenin isomer of bacopasaponin C (3) and bacopasaponin C (4) as major constituents. An HPLC method together with an optimised extraction procedure was developed for the estimation of 1–4 in B. monnieri to enable standardisation of the latter. Concentration ranges of the analytes in samples of B. monnieri collected from different regions of India were 0.14–0.85% (w/w) (1), 0.12–0.69% (2), 0.05–0.72% (3) and 0.05–0.44% (4). The importance of using bacoside A, with known concentrations of 1–4, as a reference standard for the routine analysis of B. monnieri is highlighted. Two common avonoids, luteolin and apigenin, were present in all samples of B. monnieri . Copyright © 2005 John Wiley & Sons, Ltd.