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Identifying citrus limonoid aglycones by HPLC‐EI/MS and HPLC‐APCI/MS techniques
Author(s) -
Manners Gary D.,
Breksa Andrew P.
Publication year - 2004
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.790
Subject(s) - chemistry , limonoid , high performance liquid chromatography , atmospheric pressure chemical ionization , chromatography , chemical ionization , organic chemistry , ionization , ion
Abstract HPLC coupled with normal phase electron ionisation (EI) and atmospheric pressure chemical ionisation (APCI)/mass spectrometry methods has been applied to identify 17 known neutral limonoid aglycones from Citrus sources. The HPLC‐MS data from the known limonoids provided chromatographic characteristics, APCI‐derived molecular weight data and EI fragmentation data for each limonoid. EI fragmentation patterns for the limonoids were correlated with structural characteristics. The EI fragmentation patterns coupled with APCI‐derived molecular weights were utilised as a potential method by which to discern the structural character of unknown citrus limonoids. Copyright © 2004 John Wiley & Sons, Ltd.