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HPLC‐UV/PAD and HPLC‐MS n analyses of leaf and root extracts of Vismia guineensis and isolation and identification of two new bianthrones
Author(s) -
Politi M.,
Sanogo R.,
Ndjoko K.,
Guilet D.,
Wolfender J.L.,
Hostettmann K.,
Morelli I.
Publication year - 2004
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.788
Subject(s) - chemistry , anthraquinones , anthraquinone , high performance liquid chromatography , dichloromethane , chromatography , clusiaceae , imperatorin , botany , organic chemistry , solvent , biology
In order to evaluate the possible use of the leaves instead of the roots of Vismia guineensis as a new source for the traditional use of this drug, the chemical composition of both organs were compared by HPLC‐UV/PAD and HPLC‐MS analyses. The leaves are analysed here for the rst time. The results show the presence of ve major classes of secondary metabolites having specic chromophores: anthraquinones, vismiones, avonoids, xanthones and benzophenones. The molecular weights and characteristic fragments, compared with previous EI or HPLC‐MS literature data, allowed the partial identication of the major peaks in the chromatograms. Six additional isomeric bianthrones and one anthraquinone were detected in the dichloromethane extract of the roots after long storage in solution; the targeted isolation of the bianthrones was performed and enabled the identication of two original C‐geranyl derivatives. The chemical compositions of the extracts demonstrated that only a minority of the constituents is shared by both organs. Thus, in order to establish a denitive phytoequivalence, additional pharmacological investigations are required. Copyright © 2004 John Wiley & Sons, Ltd.