Premium
Determination of isopropylmethylphenols in black seed oil by differential pulse voltammetry
Author(s) -
Michelitsch Astrid,
Rittmannsberger Anna,
Hüfner Antje,
Rückert Ulla,
Likussar Werner
Publication year - 2004
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.785
Subject(s) - chemistry , carvacrol , thymol , differential pulse voltammetry , chloride , detection limit , potassium , analytical chemistry (journal) , voltammetry , chromatography , nuclear chemistry , cyclic voltammetry , electrochemistry , electrode , essential oil , organic chemistry
Abstract A differential pulse voltammetric method is presented for the determination of isopropylmethylphenols (carvacrol and/or thymol) in phytotherapeutic black seed oils. The voltammetric behaviours of these phenols were examined in various buffer systems over the pH range 3.5–10.0. In Sörensen buffered methanol solution (3:7; v:v; pH 8.5), the differential pulse voltammograms exhibited reproducible peaks at E p + 0.49 V vs. silver–silver chloride–potassium chloride 3 m for both carvacrol and thymol. Under these conditions, a plot of peak height against concentration of the isopropylmethylphenols was found to be linear over the range 0.25–2.5 µg/mL ( r = 0.999). The detection limit was 0.04 µg/mL. The described voltammetric method was tested on two black seed oils available on the Austrian market. Copyright © 2004 John Wiley & Sons, Ltd.