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Isoflavonoids in the rutaceae family: 1. Fortunella obovata , Murraya paniculata and four Citrus species
Author(s) -
Lapčík Oldřich,
Klejdus Bořivoj,
Davidová Michaela,
Kokoška Ladislav,
Kubáň Vlastimil,
Moravcová Jitka
Publication year - 2004
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.781
Subject(s) - daidzin , isoflavonoid , genistin , chemistry , daidzein , rutaceae , biochanin a , formononetin , phytochemical , isoflavones , traditional medicine , glycitein , genistein , botany , flavonoid , biochemistry , biology , medicine , antioxidant , endocrinology
Several types of compounds with immunoreactivity similar to isoavonoids were detected in water: ethanol extracts of leaves of Fortunella obovata Hort. ex Tanaka, Murraya paniculata Jack. and four Citrus species, namely C. aurantium L., C. grandis Osbeck, C. limonia Osbeck., and C. sinensis Osbeck ( Rutaceae ). The chromatographic mobilities of the immunoreactive substances were compared with those of authentic standards, revealing a spectrum of isoavonoid metabolites in all plants studied. Aglycones as well as glycosides were recognized, namely daidzin, genistin, daidzein, genistein, formononetin, biochanin A, prunetin, and several incompletely characterized isoavonoids. A subsequent HPLC‐MS study veried the identities of the main immunoreactive isoavonoids and established the identities of several others, viz. glycitein, glycitin, ononin and sissotrin, including the malonylated and acetylated isoavonoid glucosides. The estimated content of the individual immunoreactive entities ranged from a few µg to about 2 mg/kg (dry weight). It is concluded that the isoavonoid metabolic pathway is present throughout the Rutaceae family. Copyright © 2004 John Wiley & Sons, Ltd.