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Comparison of extraction methods for secologanin and the quantitative analysis of secologanin from symphoricarpos albus using 1 H‐NMR
Author(s) -
Kim Hye Kyong,
Choi Young Hae,
Luijendijk Teus J. C.,
Rocha Ronald Armando Vera,
Verpoorte Robert
Publication year - 2004
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.776
Subject(s) - chemistry , extraction (chemistry) , chromatography , yield (engineering) , sonication , proton nmr , methanol , stereochemistry , organic chemistry , materials science , metallurgy
In order to develop an efcient large‐scale extraction of secologanin from Symphoricarpos albus , different methods have been compared. Ultrasonication with organic solvents and water, microwave‐assisted extraction and hot water extraction methods were evaluated for their efciencies. Among the methods tested, ultrasonication with methanol showed the highest yield of secologanin (3.35 ± 0.24 mg/g fresh weight). For reliable quantication of secologanin a 1 H‐NMR method was developed. The experiment was performed by the analysis of the integral of the signal of H‐9, which was well separated in the range δ 7.4–7.5 in the 1 H‐NMR spectrum. The quantity of the compound was calculated from the relative ratio of intensity of the target peak to the known amount of internal standard, 200 µg of gallic acid. This method allows rapid and simple quantication of secologanin in 5 min without any pre‐purication steps. Copyright © 2004 John Wiley & Sons, Ltd.