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Standardisation of Gymnema sylvestre R. Br. with reference to gymnemagenin by high‐performance thin‐layer chromatography
Author(s) -
Puratchimani V.,
Jha S.
Publication year - 2004
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.763
Subject(s) - gymnema sylvestre , chemistry , chromatography , silica gel , thin layer chromatography , chloroform , methanol , high performance thin layer chromatography , analytical chemistry (journal) , traditional medicine , organic chemistry , medicine
A simple and reproducible HPTLC method for the determination of gymnemagenin (1) in Gymnema sylvestre has been developed. Components were separated on pre‐coated silica gel 60 F 254 plates with chloroform:methanol (9:1) and scanned using a densitometric scanner in the UV reectance mode at 290 nm. Linearity of determination of 1 was observed in the range 4–10 µg. The average percentage recovery of 1 from an extract was 99.09 ± 0.29, and the content of 1 in leaves of the title plant was 1.61% (dry weight). Copyright © 2004 John Wiley & Sons, Ltd.