Standardisation of  Gymnema sylvestre  R. Br. with reference to gymnemagenin by high‐performance thin‐layer chromatography | Zendy

Puratchimani V. | Zendy; Jha S. | Zendy

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Standardisation of Gymnema sylvestre R. Br. with reference to gymnemagenin by high‐performance thin‐layer chromatography

Author(s) -

Puratchimani V.,

Jha S.

Publication year - 2004

Publication title -

phytochemical analysis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.574

H-Index - 72

eISSN - 1099-1565

pISSN - 0958-0344

DOI - 10.1002/pca.763

Subject(s) - gymnema sylvestre , chemistry , chromatography , silica gel , thin layer chromatography , chloroform , methanol , high performance thin layer chromatography , analytical chemistry (journal) , traditional medicine , organic chemistry , medicine

A simple and reproducible HPTLC method for the determination of gymnemagenin (1) in Gymnema sylvestre has been developed. Components were separated on pre‐coated silica gel 60 F 254 plates with chloroform:methanol (9:1) and scanned using a densitometric scanner in the UV reectance mode at 290 nm. Linearity of determination of 1 was observed in the range 4–10 µg. The average percentage recovery of 1 from an extract was 99.09 ± 0.29, and the content of 1 in leaves of the title plant was 1.61% (dry weight). Copyright © 2004 John Wiley & Sons, Ltd.

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