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Combined analysis of the essential oil of Chenopodium ambrosioides by GC, GC‐MS and 13 C‐NMR spectroscopy: quantitative determination of ascaridole, a heat‐sensitive compound
Author(s) -
Cavalli JeanFrançois,
Tomi Félix,
Bernardini AntoineFrançois,
Casanova Joseph
Publication year - 2004
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.761
Subject(s) - chemistry , gas chromatography–mass spectrometry , chromatography , carbon 13 nmr , mass spectrometry , organic chemistry
A commercial sample of the essential oil of Chenopodium ambrosioides L. from Madagascar was analysed by GC, GC‐MS and 13 C‐NMR. By GC analysis, the major constituents were found to be ascaridole (1) (41.8%), isoascaridole (2) (18.1%), p ‐cymene (16.2%), α ‐terpinene (9.7%) and limonene (3.8%). However, ascaridole undergoes a partial thermal isomerisation to 2 and hence the amount of 1 is under‐estimated by GC analysis. The actual contents of 1 and 2 (55.3 and 4.6%, respectively) were obtained following combined analysis of the sample by GC and 13 C‐NMR. Several hydroxy‐ and polyhydroxy‐menthanes were identied by 13 C‐NMR. Copyright © 2004 John Wiley & Sons, Ltd.