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Quantitative determination of curcuminoids in Curcuma rhizomes and rapid differentiation of Curcuma domestica Val. and Curcuma xanthorrhiza Roxb. by capillary electrophoresis
Author(s) -
Lechtenberg Matthias,
Quandt Bettina,
Nahrstedt Adolf
Publication year - 2004
Publication title -
phytochemical analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 72
eISSN - 1099-1565
pISSN - 0958-0344
DOI - 10.1002/pca.759
Subject(s) - curcuma , chemistry , zingiberaceae , chromatography , rhizome , capillary electrophoresis , paeoniflorin , sodium hydroxide , traditional medicine , high performance liquid chromatography , organic chemistry , medicine
Abstract The three major curcuminoids, curcumin, demethoxycurcumin and bis‐demethoxycurcumin, from Curcuma domestica Val. ( Curcuma longa L.) and Curcuma xanthorrhiza Roxb. (Zingiberaceae) were fully separated and quantied in less than 5 min using a capillary zone electrophoresis method with standard fused‐silica capillaries and photodiode array detection. An electrolyte solution of 20 m m phosphate, 50 m m sodium hydroxide and 14 m m β ‐cyclodextrin was found to be appropriate. Quantication was performed with 3,4‐dimethoxy‐ trans ‐cinnamic acid as internal standard, and the limit of detection was 0.01 mg/mL. Extraction, stabilisation during sample storage and quantication procedures were optimised and carried out with drugs and commercial curry powder from different provenances. The results were compared with the photometric method of the monograph Curcumae xanthorrhizae rhizoma of the European Pharmacopoeia . Copyright © 2004 John Wiley & Sons, Ltd.